Do you want to see how your inhibitor or compound is affecting the rest of your model’s kinome?
Where do you invest your time? Would you miss out on anything?
The Kinomeview profiling kit allows researchers to globally discover what is going on.
CST made Motif antibodies for this purpose.
Instead of a single peptide used as the antigen, CST used peptide libraries. Certain amino acids are fixed in that library (in this case, a phosphoserine and an arginine at -3 relative to that phosphoserine). Every single peptide in the library has those two residues, and all the other residues around those two are varied.
These libraries are used as the antigen to raise antibodies, and what you end up with are antibodies that are exquisitely specific for the intended motif, but broadly reactive against many different primary amino acid sequences.
This allows the antibody to pick up previously unknown substrates since the motifs are highly conserved.
There are 16 motif antibodies and 2 secondary antibodies included in the Kinomeview Profiling kit. There is 40ul of each antibody (enough for 4 westerns blots).
The table on the right shows the different motifs (and the corresponding substrates/recognition sites) that the kit covers.
What your results could look like:
Screen your samples with all 16 motif antibodies via western blots allows users to see which motifs (therefore which signalling pathways) has the most significant differential expressions between your samples (treated vs not treated etc).
In the results above, we can see that there are no significant difference between samples in PDK1 docking motifs. However, the last two samples (highlighted in red) in both the PKA and ATM/ATR substrate blots are significantly different. This suggest that you should focus on these two motifs in your subsequent experiments. This ability to observe global signalling changes across your samples, so you never miss out what’s really going on. The same antibodies in the Kinomeview profiling kit is available in a format conjugated with immunobeads so that you can use them in PTMSCan experiments (this allows you to identify actual substrates). Kinomeview is also a great way to optimise experimental conditions (treatment dose, time course, cell line, tissue etc)
How do I redeem this offer?
Genesearch e.Freezer/ web accounts: Simply add 9812s into your cart. As you checkout, click on the “USE DISCOUNT COUPON” box. Enter the promo code “20kinome” and 20% will be taken off the list price.
Purchase Orders: Put the promo code “20kinome” onto your purchase order. Let your purchaser know to include this when they send the purchase order to Genesearch. When Genesearch receives your order, the price of the kit will be 20% less.
- Offer only valid in Australia for Genesearch customers.
- Expires 30th March 2018.
- No cash or cash equivalent.
- Offer may not be applied to existing, pending or prior orders.
- Promotion code”20kinome” must be referenced when placing order (e.Freezer, web order, P.O).
- Promotion is not available in conjunction with any other promotion or offers, and applies to standard list price.
If you have any questions, please contact firstname.lastname@example.org