BTK is involved in the B cell receptor and the NF kappa B signalling pathways. It has an important role in primary immunodeficiency, in particularly, X-linked agammaglobulinemia (XLA) disease. 

Cisbio’s phospho- and total BTK assays are based on a time-resolved (TR)-FRET sandwich immunoassay format comprising two specific anti-BTK antibodies, one labeled with Eu3+-cryptate (donor) and the other with d2 (acceptor). 

The antibodies specifically bind with BTK, and the proximity of donor and acceptor will then lead to a fluorescent TR-FRET signal.

Advantages

  • Validated on Ramos and Raji cell line lysates
  • Measure Phospho & Total from the same cell lysate
  • Pre-mix detection reagents for a single dispensing step for direct detection.
  • No washing
  • Optimized for 384-well plate formate, but can be further miniaturized or upscaled
  • More convenient and faster than ELISA or WB!

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