The Quick Dephosphorylation Kit contains Quick CIP, a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5´ and 3´ ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo- and deoxyribonucleoside triphosphates (NTPs and dNTPs). Quick CIP is useful in many applications that require the dephosphorylation of DNA or RNA ends. In cloning, dephosphorylation prevents re-ligation of linearized plasmid DNA. The enzyme can quickly dephosphorylate 5´ protruding, 5´ recessed, and blunt ends in just 10 minutes. Quick CIP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis.

Quick CIP is completely and irreversibly inactivated by heating it at 80°C for 2 minutes, unlike wild type CIP, which is heat-inactivatable. This makes removal of Quick CIP prior to ligation or end-labeling unnecessary.

 

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