Start your next ChIP or ChIP Seq experiment with quality chromatin with CST’s Simple ChIP kits.

Simple ChIP kits has all the reagents, buffers, antibodies and primers you’ll need from start to finish:

Glycine Solution (10X)1 x 100 ml4°C
ChIP Sonication Cell Lysis Buffer (2X)1 x 21 ml4°C
ChIP Sonication Nuclear Lysis Buffer1 x 21 ml4°C
ChIP Buffer (10X)1 x 20 ml4°C
ChIP Elution Buffer (2X)1 x 2.5 ml4°C
5 M NaCl1 x 3 ml4°C
ChIP-Grade Protein G Magnetic Beads 90061 x 780 µl4°C
Protease Inhibitor Cocktail (200X)1 x 850 µl-20°C
RNAse A (10 mg/ml)1 x 50 µl-20°C
Proteinase K (20mg/ml)1 x 100 µl-20°C
SimpleChIP® Human RPL30 Exon 3 Primers 70141 x 150 µl-20°C
SimpleChIP® Mouse RPL30 Intron 2 Primers 70151 x 150 µl-20°C
Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated) 46201 x 100 µl-20°C
Normal Rabbit IgG 27291 x 50 µl-20°C
DNA Binding Buffer1 x 30 mlRT
DNA Wash Buffer (add 4x volume ethanol before use)1 x 6 mlRT
DNA Elution Buffer2 x 1 mlRT
DNA Purification Columns and Collection Tubes

Results:

Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT 116 cells and either TCF4/TCF7L2 (C48H11) Rabbit mAb #2569, Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb #8814,CBP (D9B6) Rabbit mAb #7425, or Normal Rabbit IgG #2729 using SimpleChIP® Plus Sonication Chromatin IP Kit #56383. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human CaMK2D Intron 3 Primers #5111, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

SPECIAL OFFER!

Order any SimpleChIP Chromatin IP Kit listed below, and you will receive a FREE SimpleChIP Universal qPCR Master Mix!

Use the promo code CHIP18 at your eFreezer, on your web order, or PO

 

 

 

Eligible SimpleChIP Chromatin IP Kits

SimpleChIP® Plus Sonication Chromatin IP Kit (Magnetic Beads) #56383

SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005

SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) #9004

SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads) #9002

SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003

 

Offer only valid in Australia for Genesearch customers. Expires September 30, 2018. Offer may not be applied to

existing, pending, or prior orders. Promotion code “CHIP18” must be referenced when placing order.

Promotion is not available in conjunction with any other promotions or offers.