Start your next ChIP or ChIP Seq experiment with quality chromatin with CST’s Simple ChIP kits.
Simple ChIP kits has all the reagents, buffers, antibodies and primers you’ll need from start to finish:
|Glycine Solution (10X)||1 x 100 ml||4°C|
|ChIP Sonication Cell Lysis Buffer (2X)||1 x 21 ml||4°C|
|ChIP Sonication Nuclear Lysis Buffer||1 x 21 ml||4°C|
|ChIP Buffer (10X)||1 x 20 ml||4°C|
|ChIP Elution Buffer (2X)||1 x 2.5 ml||4°C|
|5 M NaCl||1 x 3 ml||4°C|
|ChIP-Grade Protein G Magnetic Beads 9006||1 x 780 µl||4°C|
|Protease Inhibitor Cocktail (200X)||1 x 850 µl||-20°C|
|RNAse A (10 mg/ml)||1 x 50 µl||-20°C|
|Proteinase K (20mg/ml)||1 x 100 µl||-20°C|
|SimpleChIP® Human RPL30 Exon 3 Primers 7014||1 x 150 µl||-20°C|
|SimpleChIP® Mouse RPL30 Intron 2 Primers 7015||1 x 150 µl||-20°C|
|Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated) 4620||1 x 100 µl||-20°C|
|Normal Rabbit IgG 2729||1 x 50 µl||-20°C|
|DNA Binding Buffer||1 x 30 ml||RT|
|DNA Wash Buffer (add 4x volume ethanol before use)||1 x 6 ml||RT|
|DNA Elution Buffer||2 x 1 ml||RT|
|DNA Purification Columns and Collection Tubes|
Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT 116 cells and either TCF4/TCF7L2 (C48H11) Rabbit mAb #2569, Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb #8814,CBP (D9B6) Rabbit mAb #7425, or Normal Rabbit IgG #2729 using SimpleChIP® Plus Sonication Chromatin IP Kit #56383. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human CaMK2D Intron 3 Primers #5111, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Order any SimpleChIP Chromatin IP Kit listed below, and you will receive a FREE SimpleChIP Universal qPCR Master Mix!
Use the promo code CHIP18 at your eFreezer, on your web order, or PO
Eligible SimpleChIP Chromatin IP Kits
Offer only valid in Australia for Genesearch customers. Expires September 30, 2018. Offer may not be applied to
existing, pending, or prior orders. Promotion code “CHIP18” must be referenced when placing order.
Promotion is not available in conjunction with any other promotions or offers.