
|
Pancreatic islets were isolated from C57BL_6N male mice. The islets were dispensed into tubes with KRB buffer containing either 2.8 mM or 16.7 mM glucose _5 islets_tube_ 6 tubes_condition_. After 10 minutes or 30 minutes of incubation_ the supernatants_ were collected and the insulin concentrations measured using the HTRF Ultra-sensitive insulin kit reagents.
|


