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sample
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What is your starting material
DNA
RNA template amounts of 1pg - 10ng total RNA or >0.01pg mRNA per reaction
RNA template amounts of 10pg - 100ng total RNA or >0.01pg mRNA per reaction
Do you Syber Green or Probe?
Syber Green
Probe
Do you prefer one step RT-qPCR or cDNA synthesis for down stream applications?
one-step RT-qPCR
cDNA synthesis
N/A
Which instrument do you use
Which instrument do you use
Agilent (Stratagene) - AriaMX, AriaDX
Agilent (Stratagene) - MX3000P®, MX3005P®, MX4000P®
Analytik Jena - qTOWER, qTOWER 2.x
BMS - Mic
Bio-Rad® - CFX96™, CFX384™, CFX Connect™
Bio-Rad® - Chromo4™, MiniOpticon™, Opticon™, Opticon™ 2
Bio-Rad® - iCycler®, iQ™ 5, MyiQ™
BJS - Xxpress®
Cepheid® - SmartCycler®
Eppendorf - Mastercycler® ep realplex, Mastercycler® ep realplex 2S
Fluidigm - BioMark™
Hain Lifescience - FluoroCycler® 96
IT-IS Life Science - MyGo Pro, MyGo Mini
PCRmax - Eco™
Qiagen (Corbett) - Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q
Roche - LightCycler® 480, LightCycler® 96, LightCycler® Nano
Takara - Thermal Cycler Dice® (TP800)
Techne® - PrimeQ, Quantica®
Thermo Fisher (including Applied Biosystems and Life Technologies) - 5700, 7000, 7300, StepOne™, StepOne™ plus
Thermo Fisher (including Applied Biosystems and Life Technologies) - 7500, 7500 FAST, QuantStudio™ 3, 5, 6, 7, 12k Flex, ViiA7™
Thermo Fisher (including Applied Biosystems and Life Technologies) - 7700, 7900, 7900HT, 7900HT FAST
Thermo Fisher (including Applied Biosystems and Life Technologies) - Piko Real®
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